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SARS-CoV-2 . The method of “self-collection of samples” for the detection of

CDMX. – After completing field validation and confirming its effectiveness, the “auto-sample” method for the detection of SARS-CoV-2 was ready,…

By admin , in news , at May 12, 2022 Tags: , ,

CDMX. – After completing field validation and confirming its effectiveness, the “auto-sample” method for the detection of SARS-CoV-2 was ready, using real-time PCR, Education, Science, Technology and Innovation The ministry told ( SECTEI) of Mexico City.

The capital government explained that this method has the advantage of reducing the cost of testing as well as shortening the time of results, apart from reducing the chances of infection for health workers.

This project includes a saliva test PCR (polymerase chain reaction) is the most reliable technique for the detection of pathogens and microorganisms.

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The initiative is led by researcher Victor Julian Valdés Rodriguez from the Institute of Cellular Physiology of the National Autonomous University of Mexico.UNAM), and is financed by section,

This test is safer than others, as it does not require any other person to take the sample, a single person can do it with the help of the kit.

The kit consists of a tube where the saliva will be stored, a capillary tube in which the same person takes 20 microliters of saliva and a container where it will be emptied and inside which is an inert buffer.

Thereafter, in a diagnostic laboratory, the sample will be treated to obtain the results.

Self-sample collection method for the detection of SARS-CoV-2.

This reduces the cost of testing, timing of results and reduces the chances of infection to health workers.

The project is a saliva test for PCR (Polymerase Chain Reaction), the most reliable technology for the detection of pathogens and microorganisms.

Saliva self-collection is an alternative to the nasopharyngeal swab technique, a procedure with a certain degree of complexity that requires trained personnel protected by specialized equipment.

Extraction of saliva – a substrate in which much of the SARS-CoV-2 virus is present – ​​makes this a simpler and more convenient method.

If it is not easy to obtain a nasopharyngeal sample from an adult, it is more complicated to remove it from an infant or young child.

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“One of the best plans to avoid major impacts in the pandemic was to identify cases, as it helps trace contacts, define who is sick or not, when they are contagious and when. They don’t stay. We now have antigen tests, but PCR is more reliable,” said UNAM’s doctor of biomedical sciences, Valdés Rodriguez, with a postdoctoral degree and living at Mount Sinai School of Medicine and The Black Family Stem Cell Institute, New York City.

No person is required to take the sample

The safety of the test, developed by the expert and his team, lies in the fact that it does not require a person to take the sample, it is the willing person who performs it. The recommended location for sampling is the bathroom.

The kit consists of three elements: saliva is stored in a tube, the same person takes 20 microliters of saliva with a capillary tube that empties into another container with an inert buffer inside.

Next, the sample is sterilized in a diagnostic laboratory. To do this, it is subjected to a temperature of 96ºC for ten minutes: both heat and time are conditions in which the virus does not survive.

Samples can be manipulated to continue with real-time PCR diagnosis without the need to purify genetic material, as the inactivation buffer designed by the UNAM team is compatible with PCR.

A PCR test makes it possible to specifically detect a piece of genetic material. in the matter of SARS-CoV-2, it is RNA (ribonucleic acid). If the result is positive and does not identify genetic material, the person does not transmit the infection.

SARS-CoV-2 . The method of “self-collection of samples” for the detection of

2022-05-12 14:24:38